Protection Against Rugose

 

How to detect the tomato brown rugose fruit virus?

What is the ToBRFV?

Video introduction to protection against roughness

 

OUR

EXPERTS

How is Semillas Fitó addressing the problem of the rugose virus?

 

German Anastasio

Global Crop Coordinator Tomato

info@semillasfito.com

How can biotechnology offer a solution to the problem of the rugose virus?

 

Jordi Quilis

Biotech Director

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How do you find the balance between resistance to the rugose virus and fruit quality?

 

Pablo Quijada

Breeding Director

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How do we produce virus-free seeds?

 

Víctor Sancho

Production & Operations Manager

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VARIETIES

Alcazaba

Ideal for bunches of 500 grams.

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PLANT

High vigour, medium internode, and medium-high cover. Excellent setting during the whole growing cycle. High precocity.


FRUIT

Tomato Cluster. M-size fruit, 90/100 grams in weight, with high consistency, a deep red colour, and a long shelf life.


RESISTANCES

HR: ToMV / Pf: B-D / Fol: 0, 1 / Vd / Va

IR: TYLCV / Mi / Ma / Mj / TSWV / ToBRFV

Zones:

Central Asia, Middle East, Spain, Morocco

Cadice

Excellent yield with no craking

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PLANT

Hardy, medium vigour plant. Its leaf structure allows excellent aeration of the plant especially in the autumn-winter period. Medium internodes. Its high setting capacity even at high temperatures and its long and branched clusters allow a high and continuous yield during the whole cycle. Excellent performance in the presence of ToBRFV.


FRUIT

Cherry plum medium to large size, with a consistent shape during the entire production cycle. Excellent flavour with a balanced ratio of acids and sugars. Intense and bright red colour when ripe. Bunches with evergreen, fleshy shafts, and sepals. Excellent resistance to cracking. High post-harvest preservation.


RESISTANCES

HR: ToMV / Fol: 0 / Forl / Pst

IR: TYLCV / ToBRFV

Zones:

Italy

Copacabana

Agronomy and resistances combined in a yellow cherry plum.

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PLANT

Open strong and productive plant. It presents high resistances to Cladosporium and intermediate resistance to ToBRFV.


FRUIT

Average weight of 12-14 g. very attractive fruit with a homogeneous yellow color. Tasty fruit with high brix and crunchy texture. Its firmness gives it a good shelf life and makes it ideal for mix.


RESISTANCES

HR: ToMV / Pf: A, E / Fol: 0,1 / Vd /Va

IR: TYLCV / ToBRFV

Zones:

France 

Essentia

Cherry for collections both in bouquet as in loose

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PLANT

Variety intended for all cycles, especially long cycles.
Plant of medium vigor with a great vegetative-generative balance, medium internodes and short bouquets. Homogeneous fruits that They maintain the caliber throughout the cycle and a lot of productivity both in bouquet and loose.


FRUIT

Essentia is a sophisticated heart-shaped tomato, firm fruit
and crispy and with excellent flavor, with high Brix degrees from the beginning of cultivation. Intense red color and a great taste of sweetness. Essentia, is part of our cherry tomato specialties offer.


RESISTANCES

HR: ToMV / Fol: 0

IR: TYLCV / Mi / Ma / Mj / ToBRFV

Zones:

Middle East, Spain

Ferdinand

Fast increase in Brix

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PLANT

Plant with medium vigour, with high-vigour rootstocks and pruning to two branches. Open plant structure, with fan-shaped clusters, which emerge from the plant, thus, enabling easier harvesting. Due to its fast entry into production, it is also very suitable for short cycles.


FRUIT

Cherry Plum. Its high degrees Brix give it a great flavour. The fruit has a deep red colour and a high post-harvest shelf life.


RESISTANCES

HR: ToMV / Fol: 0

IR: TYLCV / Mi / Ma / Mj / ToBRFV

Zones:

Central Asia, Middle East, Spain, Morocco

Firstige

Resistance to the rugose virus, without compromising on quality and flavour

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PLANT

Highly vigorous plant, well adapted to production at low temperatures. Intermediate resistance to the rugose virus.


FRUIT

Cherry Plum with oval fruit and smooth wall. Very clean with no cracking or micro cracking. Maintains size and length throughout the cycle. Higher flavour and degrees Brix.


RESISTANCES

HR: ToMV / Fol: 0 / Pst

IR: TYLCV / Mi / Ma / Mj / ToBRFV

Zones:

Mexico, Central Asia

Mulan

Kilos of flavour

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PLANT

Plant with medium vigour, easy flowering and setting of fruit. Adapted to autumn and spring cycles. Open plant structure,
with fan-shaped clusters and homogeneity of the fruit in the cluster.


FRUIT

Cherry plum. Stands out for its flavour, which is maintained throughout its entire production, reaching degrees Brix from the start of cultivation. Good colour and preservation.


RESISTANCES

HR: ToMV / Fol: 0, 1

IR: TYLCV / Mi / Ma / Mj / ToBRFV

Zones:

Central Asia, Middle East, Spain, Morocco

Solemio

The sweetest flavour

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PLANT

Plant with medium/high vigour, easy flowering and setting of fruit. Compact plant structure, with shorter internodes and clusters, with each cluster divided into one or three shafts.


FRUIT

Cherry plum. Great flavour reaching ºBrix from the start of cultivation. Homogeneity of the fruit in the cluster, which maintains its size throughout the entire growing cycle. Productive despite its cluster structure, due to the higher number of clusters. Good colour and preservation.


RESISTANCES

HR: ToMV / Fol:0

IR: TYLCV / Mi / Ma / Mj / ToBRFV

Zones:

Greece, East Europe, Middle East, Spain

Frequent questions

We take the ISF (International Seed Federation) definition as a reference.

High Resistance (HR): High resistant (HR) varieties highly restrict the growth and/or development of the specified pest and/or the damage it causes under normal pest pressure when compared to susceptible varieties. These plant varieties may, however, exhibit some symptoms or damage under heavy pest pressure.

Intermediate Resistance (IR): Intermediate resistance (HR) varieties restrict the growth and/or development of the specified pest and/or the damage it causes but may exhibit a greater range of symptoms or damage compared to high resistant varieties. Intermediate resistant varieties will still show less severe symptoms or damage than susceptible plant varieties when grown under similar environmental conditions and/or pest pressure. It is also important to clarify that varieties claiming the same level of resistance against a specific pest may exhibit a different resistance response due to a different genetic makeup of a variety.

In summary, intermediate resistance implies a restriction of virus growth with the possibility of some symptoms, while high resistance implies a significant restriction, although symptoms may still occur under intense pressure. Genetic variability between varieties can influence the resistance response, even if they claim the same level of resistance.

 

Jordi Quilis

Biotechnology Director

Although the range allowed by the ISF is very broad, the guidelines set by Semillas Fitó are very strict when classifying their varieties. This is because we are very aware of the high levels of investment in human and economic resources made by our tomato producers. Therefore, we will only bring to the market products with the utmost guarantees, both in terms of protection and the quality of the harvest If we take a scale from 0 to 5, where 0 represents an immune plant and 5 indicates a sensitive plant, with 1 to 5 indicating the different levels of IR, the Semillas Fitó varieties labelled as IR fall within the range of 1 to 1.5 on this hypothetical scale.

 

Jordi Quilis

Biotechnology Director

I think that using IR seed does not guarantee a healthy harvest. IR covers a very wide range of tolerances.
If a sensitive variety is represented as white and a highly resistant one as black, IR varieties can be considered the entire grayscale, with varieties that offer some tolerance that do not necessarily guarantee a healthy harvest, and others that are closer to a high level of resistance, making the producer's work much easier.

The focus should be on the fact that we have IR varieties that minimize virus symptoms to such an extent that they enable higher productivity compared to the sensitive ones.

 

Francisco Fernández Castañeda

Global Operation Manager

The protocol we have involves taking the sample from the young leaves of the plant or from the sepal. In any case,  I have attached the internal sampling protocol so you can see the calculations for a confidence level of 95% or of 99%, and the method of collection.

 

Francisco Fernández Castañeda

Global Operation Manager

As the EU Regulation stands now, ToBRFV testing is only done using a RT-PCR analysis. PCR testing for pathogen detection is an indirect technique. Therefore, if the seed has been in contact with the virus, it will produce amplification curves, even if the virus has been inactivated or eliminated by the treatments applied to the seed.

A "positive" result from a PCR test should be considered a “suspected” case, never directly confirmed as positive, even if you perform a second PCR confirmation test. The best approach is to conclude the analysis with a direct test, such as a bioassay, to verify that the "detected" virus in that seed is alive and infectious (Koch's Postulates).            

That said, and given that a PCR is very sensitive and not everyone can perform bioassays, there are instances where what are known as late amplification curves may occur. These late amplifications (false positives) can depend on: the RNA extraction kit used, the amplification MaterMix, and the expertise of the technician. For this reason, we have tried to find a cut-off point from which the sample can be considered negative, even if the PCR shows amplification. This cut-off point is normally set at CT 32 or CT 35, depending on the laboratory, but if the laboratory is strict –CT higher than 35–, if the curve is correct they consider it positive (Zero Risk). The limit of biological correlation between a positive PCR result and field infection has not yet been validated.

 

Anna Viles

Quality Control Laboratory Manager

Traditionally, breeders have relied on visual assessment of agronomic characteristics (phenotype) to select varieties, considering aspects such as yield, disease resistance, tolerance to abiotic stresses and fruit qualities (such as taste, colour, shape, etc.). However, this phenotype-based selection method is a slow and costly process that involves waiting for the whole crop cycle.

 

Today, thanks to advances in biotechnology, a more efficient approach has been introduced. The identification of molecular markers, DNA sequences associated with specific plant characteristics, has revolutionized the breeding process. Thus, from a small tissue sample, these molecular markers allow for the rapid and accurate assessment of agronomic traits of interest, even in the early stages of plant development (such as at the seed or seedling stage in the seedbed).

This approach based on molecular markers is not only a technological advancement that significantly accelerates the selection process but also contributes to obtaining varieties with a greater number of high-value agronomic traits.

 

Jordi Quilis

Biotechnology Director

 Additional materials  

Cultivation Recommendations

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Self-Hygiene Recommendations

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Production Area Recommendations

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